BIO SCI 97 Lecture 11: Lecture 11 Notes

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G1 - active gene expression and cell activity, preparation for dna synthesis. S phase - dna replication and chromosome duplication. M phase: cell division -- mitosis and meiosis. Sister chromatids - found in diploid cells. They pair during meiosis and contain the same genes, but possibly different alleles. Interphase, prophase, prometaphase, metaphase (chromosomes align randomly along the metaphase plate with the aid of the mitotic spindle), anaphase, Telophase (two daughter cells are produced by mitosis) and cytokinesis. During mitosis, each daughter cell receives a diploid (2n) complement of chromosomes. The two daughter cells of mitosis are genetically equivalent. Nucleosome - histones form the octameric nucleosome. Contains 2x h3/h4 dimers and 2x h2a/ h2b dimers. H1 is not part of the nucleosome core. Chromatin organization - core dna wrapped around the nucleosome. Histone h1 stabilizes the 30 nm fiber. Nucleosomes can block access to the dna by rna polymerase and transcription factors.

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