BIO SCI 98 Lecture Notes - Lecture 1: Adenine, Ribose, Phosphofructokinase
1 May 2016
School
Department
Course
Professor
Document Summary
Protein purificatio n: remo v e all prot ein s exc e p t one of inter e s t. According to size, binding affinity, isoelec tric point, solubility, charg e. Ion- exch a n g e w/ cation exch a n g e r s : more neg a ti v e increa s e spe e d. Differe n c e s in sign and ma g ni t u d e of net elec tric. Gel: sep a r a t e by size w/ bigg e s t comin g out first. Size exclu sion: nee d porou s carbo h y d r a t e polym e r be a d s. Colum n: solution (mobile ph a s e ) flows throu g h ma trix (sta tio n e r y pha s e )
