LIFESCI 3 Lecture Notes - Lecture 8: Semiconservative Replication, Time Point, Macromolecule
Document Summary
A: dispersive & semiconservative - b; conservative - c. Dna molecules in dispersive and semiconservative model each have 1 heavy and 1 light strand, so shows up as mixture. Conservative dna molecules have conserved parental strand and totally different daughter strand, so show up as separated bands: clicker question: (cid:894)photo of dr. fluitt"s le(cid:272)ture slide (cid:271)y author(cid:895) Shows up as a single band, because each molecule contains same proportion of heavy & light dna. There will always be the same amount of parental dna in subsequent cell division. So ratio of original parental dna in molecule decreases in each round of replication (e. g. 0. 5 in 1st round; 0. 25 in 2nd round) So dna becomes lighter as a single band. Each strand builds its opposite strand, retaining original parental dna as single strand. No (cid:373)atter ho(cid:449) (cid:373)a(cid:374)(cid:455) ti(cid:373)es (cid:449)e u(cid:374)dergo repli(cid:272)atio(cid:374), (cid:449)e"ll al(cid:449)a(cid:455)s ha(cid:448)e (cid:1006) daughter molecules that are 50% heavy. Will not lose or disperse parental dna.
