LIFESCI 3 Chapter Notes - Chapter 11.1: Plasmid, Electron Microscope, Okazaki Fragments
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Replication begins where dna polymerase is recruited then travels bidirectionally along the dna until the whole chromosome is copied. Semiconservative: each parental dna strand is used as a template to generate a new daughter strand two daughter duplexes. Each daughter duplex conserves one strand of parental dna; the other strand is completely new. Two parental strands remain together while acting as template for production of a new dna duplex. Duplex is replicated in a random patchwork. Each strand is half old half new. Grew e. coli cells for multiple generations using 15nh4 as the sole nitrogen source to uniformly label the cellular dna with 15n. Increase in mass is used to separate [15n]dna from [14n]dna in a density gradient. 15n-labeled e. coli cells were transferred to unlabeled 14n medium and allowed one round of replication then analyzed. Conservative replication: two bands of duplex dna ( one 15n and one 14n)
