MCD BIO 104AL Lecture Notes - Lecture 9: Exponential Growth, Antimicrobial Resistance, Plasmid
10 Feb 2020
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Transformation efficieny: how well the dna/bacteria can be taken up by the plasmid 10^6 cfu/ug. For every microgram of plasmid dna trasnforms, you should get around a million colonies. # of colonies (get by counting)/amount of dna (ug) (get by calculations) Normalize by multiplying 200/360 because that was how much we used for the te. Le= # white colonies/ amount of dna (mass of the vector this time but only transform of ligation vector) Stationary phase: curve flattens out, cells might be running out of nutrients and cells may be dying, exponential growth of cells (healthy), then the cells begin to die. In order to grow competent cells, must start the night before. Montior density of the culture over time and stop when they reach . 5. Incubation of competent cells in a salt solution increases the chances of uptake. We know antibiotic resistance worked because someone did it.
