MCD BIO C141 Lecture Notes - Lecture 11: Histone H4, Repressor Lexa, Sepal
15 May 2020
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Explain the genetic and molecular interactions between abaxial identity genes and adaxial identity genes. Describe how a two-component estradiol inducible system works. Recall the role of genes specifying the middle domain and how they fit in to adaxial/abaxial pathways. Adaxialized leaves, reduced proximal-distal and medial-lateral outgrowth, bigger sam 2. Zipper loss of function can lead to adaxialization. Technique description: estradiol inducible system (xve): a synthetic transcription factor system. V-vp16 from herpes simplex virus-transcriptional activation domain e. Plants bind estrogen but do not make it. Engineered transcription factor fusion can inducibly turn on your gene of interest placed downstream of. Similar to gr fusion, except requires dimerization as well as nuclear translocation. C- no, because the gene you are trying to induce needs to be transcribed. Fusion of plant transcription factor to gr receptor. Estradiol induces nuclear translocation and dimerization xve then binds. Can induce any type of gene that is then translated.
