MCD BIO CM156 Lecture Notes - Lecture 8: Inbred Strain, Human Genetics, Alkaptonuria
26 Jan 2020
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Limitations: human and mice differ in some gene identities and copy numbers (e. g. , olfactory, immune response), and likely differ in gene regulation, species differences in longevity and environment could affect disease phenotypes. Some diseases take a long time to develop. Need to give the mice certain diets in order to replicate and activate the diseases. Each inbred strain represents a unique gene pool in which naturally occurring polymorphisms have been fixed by inbreeding. Inbred strains are produced by brother-sister mating for 20 or more generations. Process leads to one fixed allele at a certain location of the allele. Some are infected, some are not (2) modification of the mouse germline: transgene random insertion (~1980"s) Transgenic mice: targeting in es cells (1980"s) Knockout (ko), knockin, tissue-specific ko: site-specific nucleases (~2010) Possible to visualize the nuclei at this stage. Us es cells to inject into the nucleus of embryo to see it"s development.
