MIMG 102 Lecture Notes - Lecture 19: Sv40, Dna Replication, Papilloma

• School: UCLA
• Department: Microbiology, Immunology, and Molecular Genetics
• Course: MIMG 102
• Professor: Dasgupta Asim

Document Summary

Transcription: tumor antigens: all expressed early, required for dna transcription regulation & viral replication. Polyoma, papilloma, adenoviruses have their own unique t antigens, some have 2 or. Regulate transition from early to late transcription. Binds to ori and non-coding control region. Focusing on sv40 t antigen: transcriptional activation & dna replication: non-coding control region (nccr) Genomic region controlling transcription and dna replication. Has tata box, upstream activator region (bound by transcriptional co-activators), enhancer elements, ori & multiple tag binding sites. Recognized by host machinery in nucleus -> express t antigen. Translation in cytoplasm; t antigen has nuclear localization signal -> back to the nucleus -> finds dna & binds t antigen binding sites. Blocks early gene transcription (sterically interferes with tata box) -> drives transcription of late mrna products (structural proteins) Binding ori -> recruit dna replication proteins -> facilitates dna replication.