ANSC 3323 Lecture Notes - Lecture 22: Transmissible Spongiform Encephalopathy, Gene Knockout, Embryonic Stem Cell
Document Summary
+ germ line transmission of es cell genome containing targeted modification. Gray mice carry targeted genes: tetraploid complementation. 2-cell embryos (2n cells) electrofused with 1-cell tetraploid embryo (4n cell) to form a mosaic blastocyst with 2n and 4n cells. Allows for one generation instead of breeding. Able to tolerate 4n because the 2n cells were microinjected; there are still some cells with. Examples of gene tagging: reporter genes for developmental studies. Examples of gene repair: patient with faulty gene can be treated with a vector carrying corrected region of gene. Why are es cells used for targeting: pluripotent. Any tissue in our body: clonal expansion. Only es cells can do this in vitro. Example in vivo cancer: homologous recombination. Need many cells for characterization and injection. Only cell type to generate a chimera pluripotent cell: true embryonic stem cells can only be derived from a mouse. Past all 4 tests for es cell verification.
