MCB 2610 Lecture Notes - Lecture 1: Cytoskeleton, Stray Light, Electromagnet
8 Dec 2017
School
Department
Course
Professor
Document Summary
Similar to phase contrast, creates image by detecting differences in refractive indices/ thickness of dif. Clsm creates sharp, composite 3d image of specimens. Clear image produced by transmitted electrons under vaccum. Can view living specimen, observe internal euk. identify bacteria. Hollow cone of light that has been reflected/refracted by specimen enters lens. Hollow cone of light passes through specimen, retarded out of phase. Uses 2 beams of polarized light to create 3d image. Exposes specimen to uv, violet, or blue light. Sharp image created by laser beams, apertures to eliminate stray light, and computer interface. Yes, dehydrated and coated with a thin film of metal. Image created by up and down movement of prove as it maintains cosntance distance and is detected. Can be used to study surfaces that do not conduct electricity well. Rapid freezing technique provides way to preserve native state.
