NURS 3110 Lecture Notes - Lecture 2: Microaerophile, Clostridium, Exponential Growth
Document Summary
Understand key components organisms require for culturing and identification leading to treatment. Different organisms are bred in different environments. Contrast types of media regarding composition and indication for use. Identify the array of conditions that impact the microbial proliferation. Nutrients: carbon source, nitrogen source, sulfur source, phosphorus source, mineral sources (mg, fe, ca, growth factors (need these, but cannot make themselves) General purpose medium: foster growth of multiple organisms, keep them vital till plated specifically. Synthetic medium for growth and maintenance of _____: can be designed to foster growth of one of many organisms. Hydrogen ion concentration (ph): prefer neutral ph. Do not thrive in human stomach bc acidic environment (denature proteins in microorganisms, inactivating function) If stomach ph altered, could lead to problems (h. pylori: a few bacteria prefer low ph (e. g lactobacillus, fungi prefer low ph (yeasts, molds) Temperature: best at ~ 37 (~ 98 f) Types of media: transport media (culturettes)
