EEMB 129 Lecture Notes - Lecture 23: Reverse Transcriptase, Polyadenylation, Plasmid
15 Nov 2017
School
Department
Course
Professor
Document Summary
Use plasmid because it has an actual primer so it can do the pcr sequencing. Doesn"t require pcr a new method for sequencing genomes at high speed and at low cost. Adapters (composed of dna sequences that we know) attaches to each ends. Bind single-stranded fragments randomly to the insider surface of the flow cell channels. On the channel, there is already primer that matches the other end of the adapter-> add unlabelled nucleotides and enzyme to initiate solid-phase bridge amplification. Enzyme incorporates nucleotides to build double-stranded bridges on the solid- phase substrate. Denaturation leaves dingle-stranded template anchored to the substrate. Several million dense clusters of double-stranded dna are generated in each channel of the flow cell. On the graph, if it matches the reference, it show no color. Haplotype a set of markers on a single chromosome that tend to be inherited together. Know that it is cc/tt instead of tc/tc.
