ANFS332 Lecture Notes - Lecture 8: Taq Polymerase, Mycobacterium Avium-Intracellulare Infection, Intracellular Parasite
22 Sep 2019
School
Department
Course
Professor
Document Summary
A method for amplifying a piece of nucleic acid (dna) the process can be done in a few hours (30-40 cycles) A method by which the amount of the pcr product can be determined. Also referred to as real time pcr as the product can be observed in real- time. Fluorescent signal increases as dna is synthesized (sybr green) The ct (cycle threshold) is the cycle at which the fluorescence signal rises above background. The products are visualized on an agarose gel. Contagious bacterial disease of the intestinal tract of cows. First described in a dairy cow in 1895 (dr. h. a. Gram positive, small (1. 5 um) slightly curved rods. High lipid component in cell walls make them acid-fast . M. paratuberculosis is an obligate intracellular parasite of mammals. It can"t transport iron so it needs to steal iron from other cells. It not only survives attack by macrophages, this bacteria replicates inside macrophages.
