BISC300 Lecture Notes - Lecture 12: Growth Medium, Microbiological Culture, Absorbance
22 May 2018
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Lecture 12: Metabolism
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• In the lab you prepared a standard curve. On X axis you plotted cell concentration
o On Y axis you plotted optical density
• O.D.600 stands for optical density at 600nm
• If O.D.600 of 0.1 is for a bacterial culture with 120 cells/mL, then O.D.600 of 0.6 is for a
bacterial culture with higher concentration of cells
• Sample B was prepared by mixing 1mL of bacterial culture with 19mL nutrient broth.
Dilution factor of Sample B is 20
• Sample B was prepared by mixing 1mL of bacterial culture with 19mL nutrient broth. Of
the four dilutions select the one that most likely is of sample C
o ½
• Select correctly completed standard curve
o C
• Obligate aerobe: needs oxygen to survive
o #2
• Catalase and peroxidase: enzymes that break down products of oxidative metabolism
o Test tube #2
Microbial Metabolism
• Catabolism
• Anabolism
• Metabolism: interactions between energy and matter
o Energy
▪ What is it
▪ What does it do
▪ Forms of
▪ What happens to it in nature
▪ Why is it relevant to life
o Matter
▪ What is it
▪ What does it do
▪ What happens to it in nature
▪ Why is it relevant to life
Laws of Thermodynamics
• 1st law of Thermodynamics: Energy cannot be created nor destroyed
• 2nd law of thermodynamics
o Physical & chemical processes proceed in such a way that the disorder of the
universe increases to the maximum possible
Microbial Cells must do work
• Chemical work: synthesis of complex molecules
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