Class Notes (905,672)
US (350,513)
UD (6,623)
MEDT (102)
MEDT220 (26)
Lecture 10

MEDT220 Lecture 10: Identification of Biological Materials

3 Pages

Medical Laboratory Sciences
Course Code
Lehman, Donald C

This preview shows page 1. Sign up to view the full 3 pages of the document.

Loved by over 2.2 million students

Over 90% improved by at least one letter grade.

Leah — University of Toronto

OneClass has been such a huge help in my studies at UofT especially since I am a transfer student. OneClass is the study buddy I never had before and definitely gives me the extra push to get from a B to an A!

Leah — University of Toronto
Saarim — University of Michigan

Balancing social life With academics can be difficult, that is why I'm so glad that OneClass is out there where I can find the top notes for all of my classes. Now I can be the all-star student I want to be.

Saarim — University of Michigan
Jenna — University of Wisconsin

As a college student living on a college budget, I love how easy it is to earn gift cards just by submitting my notes.

Jenna — University of Wisconsin
Anne — University of California

OneClass has allowed me to catch up with my most difficult course! #lifesaver

Anne — University of California
MEDT 220: 9/22/17 Friday, September 22, 201711:11 AM • Immunoassays (cont) • Immunochromatographic Test (ICT) ○ Lateral flow § Sample added to strip that contains a dye -labeled antibody □ If Ag is present, a complex forms § Buffer carries immune complex along strip to "test zone (line)" Internal control: some unbound labeled Ab moves past the test § zone to the control line ○ Less complex than ELISA ○ Field test (seminal fluid and saliva assays) ○ SERATEC PSA SEIMQUANT (PSA=prostate specific antigen) ○ PowerPoint for example of positive/negative assays § Positive result both lines are colored in § Negative result only one line (control) colored in ○ Screening test for drugs ○ Examples § HemaTrace (hemoglobin/blood) § Saliva (amylase/human saliva) § Semen (semenogelin/human semen) • Identification of Biological Materials • Preliminary (screening, presumptive) tests ○ Positive indicated blood might be present ○ Negative rules out blood ○ Can sometimes get a false positive • Confirmatory tests • Additional testing ○ DNA profiling • Sensitivity (95%) ○ Probability that the presence of an analyte will give a positive result • Specificity (85-90%) ○ Probability that the absence of an analyte will give a negative result • Blood ○ Whole blood ○ Probability that the presence of an analyte will give a positive result • Specificity (85-90%) ○ Probability that the absence of an analyte will give a negative result • Blood ○ Whole blood § Two parts □ Cellular elements (red and white blood cells (w/DNA, less dense), platelets) □ Fluid portion (serum (clotted blood) or plasma (un -clotted blood)) ○ Red blood cells § Erythrocytes § In bone marrow § Mature RBCs lack nuclei § Contain hemoglobin: made up of four polypeptides, 2 alpha and 2 beta, and one atom of iron § Hemoglobin can bind other molecules like CO □ Hypoxia: is a decrease in oxygen when it can't bind § Heme group is also found in muscle, myoglobin ○ White blood cells § Three types □ Granulocytes
More Less
Unlock Document

Only page 1 are available for preview. Some parts have been intentionally blurred.

Unlock Document
You're Reading a Preview

Unlock to view full version

Unlock Document

Log In


Don't have an account?

Join OneClass

Access over 10 million pages of study
documents for 1.3 million courses.

Sign up

Join to view


By registering, I agree to the Terms and Privacy Policies
Already have an account?
Just a few more details

So we can recommend you notes for your school.

Reset Password

Please enter below the email address you registered with and we will send you a link to reset your password.

Add your courses

Get notes from the top students in your class.