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MEDT220 (26)
Lecture 10

MEDT220 Lecture 10: Identification of Biological Materials
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3 Pages
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Department
Medical Laboratory Sciences
Course Code
MEDT220
Professor
Lehman, Donald C

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MEDT%220:%9/22/17
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Description
MEDT 220: 9/22/17 Friday, September 22, 201711:11 AM • Immunoassays (cont) • Immunochromatographic Test (ICT) ○ Lateral flow § Sample added to strip that contains a dye -labeled antibody □ If Ag is present, a complex forms § Buffer carries immune complex along strip to "test zone (line)" Internal control: some unbound labeled Ab moves past the test § zone to the control line ○ Less complex than ELISA ○ Field test (seminal fluid and saliva assays) ○ SERATEC PSA SEIMQUANT (PSA=prostate specific antigen) ○ PowerPoint for example of positive/negative assays § Positive result both lines are colored in § Negative result only one line (control) colored in ○ Screening test for drugs ○ Examples § HemaTrace (hemoglobin/blood) § Saliva (amylase/human saliva) § Semen (semenogelin/human semen) • Identification of Biological Materials • Preliminary (screening, presumptive) tests ○ Positive indicated blood might be present ○ Negative rules out blood ○ Can sometimes get a false positive • Confirmatory tests • Additional testing ○ DNA profiling • Sensitivity (95%) ○ Probability that the presence of an analyte will give a positive result • Specificity (85-90%) ○ Probability that the absence of an analyte will give a negative result • Blood ○ Whole blood ○ Probability that the presence of an analyte will give a positive result • Specificity (85-90%) ○ Probability that the absence of an analyte will give a negative result • Blood ○ Whole blood § Two parts □ Cellular elements (red and white blood cells (w/DNA, less dense), platelets) □ Fluid portion (serum (clotted blood) or plasma (un -clotted blood)) ○ Red blood cells § Erythrocytes § In bone marrow § Mature RBCs lack nuclei § Contain hemoglobin: made up of four polypeptides, 2 alpha and 2 beta, and one atom of iron § Hemoglobin can bind other molecules like CO □ Hypoxia: is a decrease in oxygen when it can't bind § Heme group is also found in muscle, myoglobin ○ White blood cells § Three types □ Granulocytes
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