CBIO 3400 Lecture 7: CBIO 3400 2:18- posted
18 Feb 2019
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Document Summary
Mad2 is a signal that is sent out. Unattached kinetochores create a platform to produce closed mad2 which is a negative regulator of cdc20: two forms of mad 2(closed & open form, closed form is inactive form. Sac: global signaling mechanism preventing mitotic exit: apc/c is an e3 ubiquitin ligase that adds ubiquitin to securin and cyclin b, makes adaptors and substrate recognition modules that fit into ubiquitin. Gives targeting specificity to the enzyme: cdc20 (cell division cycle 20) is the substrate recognition module, seperase (protease) has an inhibitor bound to it called securin (inactivating component). Microtubule stability and kinesin/dynein motors: microtubule stability- depolymeraziation/polymerization of microtubules, treadmilling, tubulin flows constantly toward the spindle poles (- ends, eividence of treatmilling, kinesin/dynein motors. Mechanics behind anaphase: microtubule stability (depolymerization: dam1 ring connects to the kinetochore of the sister chromatin and makes a ring around the microtubule.
