MCB 150 Lecture Notes - Lecture 2: Nuclear Pore, Nuclear Membrane, Cytoplasm

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Life (cid:314) requires a structural compartment separate from the external environment (cid:314) molecules can perform unique functions in a relatively constant internal environment (cid:314) life needs a place to live. Cells are the fundamental units of life. Need to be large enough to sustain metabolism. Smaller the cell (cid:314) the greater sa: (cid:314) more efficient. Resolution: the ability to identify the separation of two objects that are close to each other. Resolving power of light microscopes is ~0. 2 microns ( m; 10-6 m) Electron microscopy has resolution of ~0. 5 nm (10-9 m) Denser material (cid:314) absorbs more electrons (cid:314) appears darker lighter images mean less dense material. Light microscopes first type of microscopy that was invented. Great for seeing cells + some subcellular objects (cid:314) large organelles such as chloroplasts + mitochondria + bacterial cells and small eukaryotic cells like human rbc. electron microscopy. Smaller subcellular machines like ribosomes or individual proteins.

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