MCDB 427 Lecture Notes - Lecture 5: Dot Blot, Reverse Transcription Polymerase Chain Reaction, Reverse Transcriptase
21 Dec 2016
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Have a mixture of proteins and separate based on size on sds-page. Then blot the proteins to a film membrane (similar set up to southern and. We detect the protein with an antibody we already had. Then expose the mixture to a secondary antibody that is labeled in some way that we can detect. Then expose this and you"ll see a band. It will tell us the size of the band and the abundance of the protein we started with. Also called immunoblotting because the antibodies are made from mammals. A labeled primary antibody takes work and effort. Easier to make use of commercial available secondary antibody which will bind to any primary antibody. An example is northern blot which we covered. First group can be used to generalize the transcript level/size of the transcript. Another way to say transcript level is (cid:498)steady state transcript(cid:499)
