BIOCHEM 523 Lecture Notes - Lecture 17: Citric Acid Cycle, Oxidative Decarboxylation, Acetyl Group

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Biochem423/523 - lecture 17 - the citric acid cycle. Conversion of pyruvate to acetyl-coa is unidirectional. Acetyl-coa can be utilized in many pathways. Acetyl-coa is a product of multiple catabolic pathways. Oxidative decarboxylation and deamination of amino acids. Complete oxidation of the two carbons to co2. Generation of reducing power as nadh and fadh2. Pyruvate dehydrogenase complex: 3 enzymes & 5 coenzymes. Dehydrogenases catalyze the most common substrate oxidations, which involve the loss. Oxidation of pyruvate to acetyl-coa is a virtually irreversible multistep reaction requiring. Three enzymes - pyruvate dehydrogenase (e1), dihydrolipoamide transacetylase (e2), of hydrogen three enzymes and five coenzymes and dihydrolipoamide dehydrogenase (e3) - are involved in the five-step sequence. They are organized into a multienzyme complex called the pyruvate dehydrogenase complex, pdh complex. Nad+, fad, and coenzyme a all contain an adenosine moiety. Coa and lipoamide have thiol groups that can form thioesters. The five coenzymes that function with the pdh are the following.

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