BIOCHEM 523 Lecture Notes - Lecture 4: Nucleotide, Pyrophosphate, Ribose

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The 5" end is esterified and forms a bond. The 3" hydroxyl of the dna chain attacks the a-phosphate of a deoxynucleotide. High g content will melt at a higher temperature than a content because of the third. Base pairing gives rise to dna secondary structure. The a form is displayed under low humidity. Double-stranded rna molecules and dna-rna hybrid molecules always are in. It results because of the extra hydroxyl group on the ribose in rna, causing the a form steric hindrance and spacing or the strands. The b form, displayed under high humidity, stacks the nucleotides as much as they can (it takes 10 nucleotides to make a full turn) 36 degree rotation between adjacent bases in the chain. The 5" end is the end with a phosphate, and the 3" end is the end with the hydroxyl. The bases are in the same plane. There is only one single groove (see the image on the right)

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