BIOLOGY 151 Lecture Notes - Lecture 14: Agrobacterium Tumefaciens, Ti Plasmid, Acetosyringone
16 Nov 2017
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Plasmid - small circular, double stranded dna molecule. Opine - gene that tells plant to make food molecules for bacteria. Contains molecule that kills insect after it eats leaf. Receptor coded by virulence a gene on plasmid. Virg activates transcription of other virulence factors on. Virulence region contains virulence genes a-f (vir genes) Expression of these genes results in the transfer of the t- This causes plant cells to proliferate and produce molecules that a. tumefaciens uses for energy (opines) Vir d proteins covalently bond to 5" end of t-dna strand. Vir e proteins bind to t-dna strand a. b. Forms type-4 secretion system through form of pores a. t-dna moves through pores and into plant cell cytoplasm t-dna enters nucleus and inserts itself into plant cell genes. Oncogenes - make plant form crown gall (tumor-cell growth) Toxin binds to receptors on insect gut cells. Insect dies as gut bacteria proliferate into body.
