BIOLOGY 151 Lecture Notes - Lecture 24: Green Fluorescent Protein, Nuclear Localization Sequence, Nuclear Pore

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4 Nov 2018
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Moves proteins from the cytoplasm into the nucleus. Importin interacts with nuclear pore complex and moves into the nucleus. Rna binding protein binds to and blicks the acceptor site of intron 1 in the cdki gene. In rna processing, the next available acceptor site would be used. The functional cdki protein requires amino acids encoded in exon 3. The protein produced when exon 2 is present is only 20 amino acids long. The protein produced when exon 2 is absent is 50 amino acids long. Moving proteins within the cell or out of the cell. Released from the ribosome into the cytoplasm. Moves into nucleus through nuclear pore complex. Anchor sequence leaves part of the protein on each side of membrane. The nucleus would glow red and the cytoplasm would glow green. What would happen if a cell contained keisha"s construct that had an nls in exon 2, 5" of the gfp sequence, and cdki rna binding protein is expressed.

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