BIOL 212 Lecture Notes - Lecture 20: Nadh Dehydrogenase, Lactate Dehydrogenase, Atp Synthase

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5 Apr 2018

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Conversion pyruvate to lactate (fermentation) yields no more atp and is used to regenerate. Nad+ plus lactate dehydrogenase leads to lactate. Glucose + 2 adp + 2 pi 2 lactate + 2 atp + 2 h2o. Contains enzymes for oxidation of pyruvate and fatty acids, and enzymes of citric acid cycle. Contains mitochondrial dna, ribosomes, trnas, various enzymes required for mitochondrial gene expression. Most mitochondrial proteins encoded by nucleus and imported into mitochondrion from cytoplasm. Contains enzymes for oxidation/reduction reactions in electron-transport chain. Contains transport proteins for passage of metabolites into and out of the matrix (pyruvate transporter) Contains large channel forming protein (porin) permeable to molecules of 5000 daltons or less. Small molecule and ion composition is similar to the cell cytoplasm because of the permeability of the outer mitochondrial membrane. At synapse electrical signal converted into a chemical signal. Theory explains how membrane transport of electrons and protons (osmotic) is linked w/atp synthesis (chemi)

Related Questions

Fill in the blank in the following table:

metabolic cycle	location pro/ eukaryote	components	substrates	products	pathway purpose
embden- meyerhof- parnas (glycolysis)	cytoplasm/ cytoplasm	10 enzymes
pentose phosphate pathway	cytoplasm/ cytoplasm	dozen enzyme	1 Glucose (6 carbon), 1 NADP, 1ATP	1 Ribose-P (5 carbon) + 1CO2, 1NADPH, or sugars, 1 ADP	Nucleotide synthesis, NADPH for anabolism
PDH reaction	cytoplasm/ mitochondria matrix
TCA/ Krebs/ citric acid cycle					generation of high energy electron carriers, provide metabolic precursors
Electron transport chain + ATP synthase (aerobic respiration)
Electron transport chain + ATP synthase (anaerobic respiration)			1Nitrate or sulfate or CO2, 1NADH + ____ ADP Or 1FADH2+ ____ ADP	N2, or NO2 or H2S or CH4 1NAD + ____ ATP Or 1FADH2+ ____ ATP	Generate proton motive forces abd use to generate ATP regenerate NAD+
Fermentation	cytoplasm/ cytoplasm	1 or more enzymes (vary by organism)	1 pyruvate, 1 NADH	1 alcohol or acid (maybe 1 CO2) NAD+	Regenerate NAD+

1. Plants need which of the following to carry on photosynthesis?

a. H2O

b. CO2

c. O2

d. both O2 and CO2

e. both H2O and CO2

2. Organisms that derive their chemical energy either from the process of chemosynthesis or photosynthesis are classified as

a. autotrophs.

b. parasites.

c. heterotrophs.

d. saprophytes.

e. mutualists.

3. The carbon source for organisms that derive their energy from photosynthesis is

a. carbon monoxide.

b. carbon dioxide.

c. hydrocarbons.

d. methane.

e. glucose.

4. The oxygen released in photosynthesis comes from

a. carbon dioxide.

b. glucose.

c. ribulose bisphosphate.

d. water.

5. When chlorophyll a molecules absorb energy, the molecules

a. release electrons.

b. release X-rays.

c. release energy.

d. release electrons and X-rays.

e. release electrons and energy.

6. The flow of what particle across the thylakoid membrane powers the production of ATP?

a. electrons

b. hydrogen ions

c. oxygen

d. carbon dioxide

e. phosphate ions 2

7. The light-independent reactions were discovered by

a. M. D. Hatch.

b. Andrew Benson.

c. Melvin Calvin.

d. Robert Hill.

e. both Andrew Benson and Melvin Calvin.

8. For each six atoms of carbon dioxide fixed in the light-independent reactions, how many molecules of PGAL (phosphoglyceraldehyde) are produced?

a. 2

b. 3

c. 6

d. 12

e. 15

Chapter 7

9. The ultimate source of energy for living things is

a. the Krebs cycle.

b. fossil fuels.

c. the sun.

d. glycolysis.

e. aerobic respiration.

10. When molecules are broken apart in respiration

a. the heat produced is used to drive biological reactions.

b. the oxygen in the compounds that are broken apart is used as an energy source.

c. the energy released in respiration is channeled into molecules of ATP.

d. ATP is converted into ADP.

e. ADP is released as a waste product.

11. ATP

a. can be produced by photosynthesis.

b. is produced in the degradation of organic compounds such as glucose.

c. is generated in anaerobic respiration.

d. is released in aerobic respiration.

e. all of these

12. Which part of the energy-releasing pathway can convert all carbon that enters to carbon dioxide?

a. fermentation

b. glycolysis

c. Krebs cycle

d. electron transport

e. phosphorylation

13. Which liberates the most energy in the form of ATP?

a. aerobic respiration

b. anaerobic respiration

c. alcoholic fermentation

d. lactate fermentation

e. All liberate the same amount, but through different means.

14. Aerobes use ________ as the final electron acceptor in electron transport phosphorylation.

a. hydrogen

b. carbon

c. oxygen

d. H2O 3

15. Glycolysis depends on a continuous supply of

a. NADP.

b. pyruvate.

c. NAD+.

d. NADH.

e. H2O.

16. Glycolysis

a. occurs in the mitochondria.

b. results in the production of pyruvate.

c. occurs in the cytoplasm.

d. occurs in the mitochondria and results in the production of pyruvate.

e. results in the production of pyruvate and occurs in the cytoplasm.

17. The end product(s) of glycolysis is(are)

a. acetyl CoA.

b. carbon dioxide.

c. pyruvate.

d. glucose.

e. glucose and carbon dioxide.

18. Which is capable of being reduced during both glycolysis and the Krebs cycle?

a. NAD+

b. FAD+

c. ADP

d. NADH

e. NADP+

19. The Krebs cycle takes place in the

a. ribosomes.

b. cytoplasm.

c. nucleus.

d. mitochondria.

e. chloroplasts.

20. The breakdown of pyruvate in the Krebs cycle results in the release of

a. energy.

b. carbon dioxide.

c. oxygen.

d. energy and oxygen.

e. energy and carbon dioxide.

ochremole959

QUESTION 1

Which of the following is the correct order of the three stages of cellular respiration?

	a.	Krebs cycle, glycolysis, electron transport
	b.	electron transport, glycolysis, Krebs cycle
	c.	Krebs cycle, electron transport, glycolysis
	d.	glycolysis, Krebs cycle, electron transport
	e.	electron transport, Krebs cycle, glycolysis

1 points

QUESTION 2

Consider a bacterial cell that performs anaerobic respiration. If that bacterial cell had access to six molecules of glucose to use, how many ATP molecules would it be able to produce?

	a.	2
	b.	34
	c.	36
	d.	6
	e.	12

1 points

QUESTION 3

The oxygen that organisms need to live is used exclusively in which of the following metabolic pathways?

	a.	Electron transport chain
	b.	Anaerobic respiration
	c.	Glycolysis
	d.	Krebs cycle
	e.	ATP-Synthase

1 points

QUESTION 4

The electron transport chain uses the energy in NADH and FADH₂ to pump H⁺ ions from the matrix to the innermembrane space. The H⁺ gradient is then used to power ATP-Synthase. Enough H⁺ is pumped from the energy in a molecule of NADH to produce ______ ATP molecules, while enough H⁺ is pumped from the energy in a FADH₂ molecule to produce ______ ATP molecules.

	a.	three; two
	b.	three; three
	c.	two; two
	d.	two; three

1 points

QUESTION 5

Which of the following list contains only products of cellular respiration?

	a.	glucose, oxygen, ADP
	b.	ATP, carbon dioxide
	c.	oxygen, ADP, carbon dioxide
	d.	ATP, glucose, oxygen
	e.	glucose, ATP

1 points

QUESTION 6

What powers the enzyme ATP-Synthase?

	a.	NADH molecules
	b.	oxygen (O₂)
	c.	ATP
	d.	NADH and FADH₂ molecules
	e.	The H⁺ gradient between the matrix and inner-membrane space

1 points

QUESTION 7

Organisms exist which are able to survive using only glycolysis (i.e. without using oxidative phosphorylation).

True

False

1 points

QUESTION 8

Each ADP molecule contains ________ phosphates, and each ATP molecule contains ________ phosphates.

	a.	three; three
	b.	two; two
	c.	one; two
	d.	two; three
	e.	one; three

1 points

QUESTION 9

Adding a phosphate group to ADP to form ATP is an endergonic reaction.

True

False

1 points

QUESTION 10

The majority of ATP produced during cellular respiration is produced during glycolysis.

True

False

1 points

QUESTION 11

Glycolysis occurs:

	a.	on enzymes located in the inner-membrane.
	b.	in the cytosol.
	c.	in the inner-membrane space.
	d.	in the nucleus of the cell.
	e.	in the matrix of mitochondria.

1 points

QUESTION 12

Muscle soreness associated with strenuous exercise is due to:

	a.	the production of alcohol during anaerobic respiration.
	b.	a buildup of lactic acid.
	c.	an excessive amount of ATP that builds up during exercise.
	d.	the large amounts of CO₂ building up in the muscle.

1 points

QUESTION 13

A required reactant for glycolysis is:

	a.	the electron transport chain.
	b.	carbon dioxide.
	c.	oxygen.
	d.	NAD+.
	e.	mitochondria.

1 points

QUESTION 14

NADH is produced in all of the following except:

	a.	the electron transport chain.
	b.	the Krebs cycle.
	c.	glycolysis.

BIOL 212 Lecture Notes - Lecture 20: Nadh Dehydrogenase, Lactate Dehydrogenase, Atp Synthase

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