BMEN 2501 Lecture Notes - Lecture 1: Differential Interference Contrast Microscopy, Bright-Field Microscopy, Histology
Document Summary
Bmen 2501 lectures notes from 9/7/16 and 9/9/16. Ch 1: cells: the fundamental units of life. Associate accurate scale with cell and molecular features. Determine resolution limit of a given microscopy system. Select proper imaging system for a given scenario. Fixation: to preserve a specimens components and volume and prevent decomposition. Resolution limit is the distance/size of the feature seen. Na= n sin which is given on most microscopes. N = refractive index of media ex. air or water. = half the angular width of the cone. Instruments: light: bright field, phase contrast and differential interference contrast, fluorescence, confocal, and multiphoton, electron: transmission, scanning, tomography. Look @ intact microtissue to create 3d digital reconstruction. Macromolecular distribution in cells in culture or cells of tissue. When cell/tissue viability is essential in vivo. Submicron features/characteristics of cells or histologic sections of tissue.
