ANAT 262 Study Guide - Midterm Guide: Glycosyl Acceptor, Emerin, Progerin

• School: McGill University
• Department: Anatomy & Cell Biology
• Course: ANAT 262
• Professor: John Presley

Document Summary

Important 1950s => em dominant from 1950s-1970s => yeast genetics 1980s to present. Antony van leeuwenhoek: apparatus single magnifying lenses, position and focus could be adjusted. Nanometer scale = em: details such as bilayer, diameter axon/microtubules. Conventional microscope: light source goes through sample, lens and then camera. Types: (1) transmitter light (brightfield, phase-contrast), (2) fluorescence, (3) confocal: most specimen very thin. To make them visible: stain with dyes or use special optics to enhance contrast: advantage unstained live cells: allow prolonged observation live cells (e. g. movements in cell division) if filmed, disadvantage unstained live cells: impossible to localize poi. Disadvantage: cannot do this indefinitely, cells undergo senescent (telomere) Contact inhibition: formation monolayer, stop growing (e. g. need to know how certain kind of cell operates) Immortalized cell: mutations involved expression telomerase, do not undergo cell crisis, still contact inhibition properties (cell line from 1 cell) (e. g. general characteristic of a cell) Transformed cell: immortalized and no contact inhibition.