CHEN 3701 Lecture 33: 2015_11_6 Lecture
Document Summary
Cell growth: introduce dna/engineer cell to produce protein/biomolecule of interest, grow cells to high density (focus of today"s lecture, induce expression of protein/biomolecule of interest, isolate fraction of interest, further purify molecule from that fraction, quality control. -> growth rate = death rate death phase: growth rate < death rate. X refers to the viable phase density (when cell dies, it doesn"t count towards total) Chen3701_biomolecularengineering page 1 not all cells double at same timeat certain time, a certain number of cells are doubling. Subexponential growth we already mentioned death or lack of nutrients causing limit in exponential growth. A linear decrease in growth rate) not mechanistic, rather emperical) as cell density gets higher, can"t maintain same level of growth. K = carrying capacity for t --> infinity, n --> k for k >> n0 exponential as expected mu is implicitly linked to time via concentration.