BIOL 483 Lecture Notes - Lecture 56: Zygote, Laplace Expansion, Immunostaining
Document Summary
Plants can transmit epigenetic info through transgenerational means. Animals transmit genomic imprinting patterns through different means. Conside tissue psecificity of methylations, combination of methylated and unmethylated sites are huge. Demethylation can provide very information rich gene regulation mechanism. Close correlation between age dependent dna methylation changes and abilities to predict human biological age. How to use tech to locate demethylation across genome. Promoter in front of germline specific gene - gene turned on when c not methylated, turned off if somatic cells and most of the cpg are methylated. Detection techniques of dna methylation: methylation sensitive restriction enzyme, affinity purification, bisulphite sequencing = gold standard, explores differential chemical activity between sodium bisulphite with methylated/ unmethylated cpg site, when c methylated it will stay as c. How does data (wgbs) helps and how it"s visualised across genome browser and how can they help us interpret correlation between dna methylation and other histone modfifications or genome expression state.
