BIOL 483 Lecture Notes - Lecture 93: Immunoprecipitation, Reference Genome, Restriction Enzyme
Document Summary
Nucleus is 6 micrometre, dna that we try to pack in is 2m long (packing 333k shirts into a suitcase) Efficient packaging means that if at any time you need to take out a tshirt, must take out specific tshirt at right time. When certain stimuli activates cell or cell differentiation and transcribing games, must be able to do this in quick way to respond to certain stimuli like neurons that happen in order of seconds. Other challenges - when cells replicate and divide, must be able to replicate into 2 daughter cells with genetic material and have to be super accurate. Fortunately, nature already figured out how cells deal with these problems and do efficient packaging. In last couple of years, 2 major technologies have been used to understand the 3d genome folding and architecture in nuclei - 1) microscopy based approach 2) chemical cross linking and proximity ligation based methods (used more recently)
