BIOL 522 Lecture Notes - Lecture 3: Bacterial Artificial Chromosome, Nanopore Sequencing, Shotgun Sequencing
Document Summary
Get 700bp, needs primers, won 1977 nobel prize: shotgun sequencing. Take whole genome and blow it up (w/ e. coli) into a bunch of different parts and sequence everything and hope that you can align parts, bac to bac (bacterial artificial chromosome - vector) This was how human genome sequencing was done. Hope that bacs are contiguous and build up until you get a contig/ get whole genome - won"t work if you don"t have a reference genome. Read light coming off of circulating dumbbell bases. Use this in combination with luminescence sequencing but it can put together bigger chunks: illumina sequencing. Pcr but locationally restricted, then cleave off one end, hopefully clones with the original. Each step can introduce bias: oxford nanopore. Can we sequence without __, real time, takes advantage of the nucleotides" electrical signal. Nanopore sequencing is a unique, scalable technology that enables direct, real-time analysis of long.
