1. Haploid Saccharomyces cerevisiae yeast cells contain the following alleles at the MAT locus.
a. Allele a
b. Allele alpha
c. Allele a or alpha
d. Alleles a and alpha
2. The following statements are true for the Saccharomyces cerevisiae yeast, except __________.
a. Yeast mating type a produces a-factor.
b. Yeast mating type alpha produces alpha-factor.
c. a-factor or alpha-factor signals the presence of a yeast cell to the neighboring cells.
d. Yeast mating type a-cell responds to a-factor, alpha-cell responds to alpha-factor.
3. A yeast strain can sexually conjugate with another yeast strain of the same mating type.
a. True
b. False
4.Heterothallic Saccharomyces cerevisiae yeast strains commonly used in controlled experiments are haploid, with either mating type a or alpha.
a. True
b. False
5. Complementation test is used to ____________________________.
a. determine the mating type of yeast.
b. determine whether mutations occur at the same gene or different genes.
c. identify mutant organisms.
d. determine functional relationships between genes.
6. Yeast cells with mating type alpha can sexually conjugate with yeast _________.
a. mating type a
b. mating type alpha
c. allele a or alpha
d. alleles a and alpha
7. Complementation test is conducted by ___________ two strains of organisms with different mutations.
a. isolating
b. morphologically identifying
c. growing
d. intercrossing
8. Assuming alleles B or b are found in locus 1; alleles C or c are found in locus 2. Which of the yeast strains below contains recessive mutations at loci 1 and 2?
a. Yeast strain with alleles B and C
b. Yeast strain with alleles B and b
c. Yeast strain with alleles C and c
d. Yeast strain with alleles b and c
9. When mutations occur at different genes, two strains of yeast are considered to be not complement.
a. True
b. False
10. Yeast cells that are ready to mate will produce a mating projecting called _______ to form a pear-shape cell.
a. pilus
b. yeast ring
c. shmoo
d. spore