BIO 320 Lecture Notes - Lecture 4: Clathrin, Atp Hydrolysis, Hydrolysis
Document Summary
Following folding and modifications in the er, proteins are sent to the (cis) golgi. The proteins make their way through the golgi compartments and are sorted at the trans-golgi. Proteins and molecules move along the secretory pathway via vesicular trafficking. Donor membrane buds off into a transport vesicle, and then the vesicle fuses with the target membrane. Only vesicles with the correct cargo are allowed to fuse! *before talking about budding, it is important to understand gtpases and how they regulate like molecular switches. Gtpase: a protein that binds to gtp and hydrolyzes it to gdp + pi these proteins hydrolyze. Gtp on their own, however it might be very slow. These gtpases, when bound to gtp are active (molecular switch turned on), and bind to effector proteins that then become activated: the slow gtpase activity of the protein turns off its ability to bind to its effector proteins. Gap: gtpase-activating protein: these proteins stimulate the hydrolysis of gtp.
