BCHM 3114 Lecture Notes - Lecture 3: Sickle-Cell Disease, Torr, Partial Pressure
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Learning goals: protein ligand binding: write the equilibrium for the binding of one ligand molecule (l) to a monomeric protein (p) and define the dissociation constant, kd. L (ligand) + protein (protein) l * p (protein binds the ligand in a cleft or groove) = [l][p] / [l * p] (units of m) Fractional saturation is when the proportion of the total myoglobin molecules have bound to o2. Half of the fractional saturation linked to the [l] gives the kd (50% of the binding receptors are occupied by a ligand: describe the structural feature of proteins that enables ligand binding and define the term saturation . Saturation means that as the oxygen concentration increases, more and more oxygen can bind to the heme group of myoglobin molecules until at a very high concentration of oxygen until all the molecules have bound o2. Proteins have the active site that allows the ligand to bind to.
