CHEM 2545 Lecture Notes - Lecture 3: Ethyl Acetate, Methyl Benzoate, Hexane
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State the objective(s) of the lab (2 pts) The goal of this lab was to use thin layer chromatography to separate the pigments present in a spinach leaf. Another portion of this lab was done to create a solvent that successfully separates different solutions of benzophenone, methyl benzoate, biphenyl, and triphenylmethanol through thin layer chromatography. Give a summary of your observations (15 pts) The first part of the experiment had to do with performing a thin layer chromatography sample on a solution made from spinach leaf. This solution was spotted on three separate tlc plates, each with different solvents including one containing 100% hexane, 100% ethyl acetate, and one with 75%/25% hexane and ethyl acetate. The results for the sample with 100% hexane include a single large spot less than a quarter of the way up the tlc plate. The second sample with a solvent system containing 100% ethyl acetate contained two distinct spots, one with an rf factor of.
