PSY 320 Lecture Notes - Lecture 4: Mass Spectrometry, Rna Interference, In Situ Hybridization
Document Summary
Factors: rna polymerase binds to the promoter and dna unwinds at the beginning of a gene. Once base pairing is completed rna polymerase is release and dna helicase is then reset: protein folds and isn"t a straight line because of polarity and charge, dna==mrna==protein, dna. If you want to know the genome, can take a sample and instead of sequencing it, looks to see if the mutant lacks or has a gene look just for the mrna. You could inactivate the gene- flox allows manipulation. Can add or insert genes. (could be from completely different species. ) Includes a reporter gene that allows a particular area to glow. If that gene becomes active, the gene for reporter will become active and glow. Ex: have gene of interest and add more copies of it: mrna transeriptome- all possible genomes that could be produced. Can have particular gene, create mrna from it.
