BIO 2110 Lecture Notes - Lecture 1: Arginine, Tata Box, Helicase

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9 Oct 2016

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Bio 2100 exam 1 study guide: shown below is a diagram of a nucleotide monophosphate. Also, on the diagram, indicate the positions of the 5" and 3" carbons. This is a deoxyribose nucleotide, which is indicated by the absence of the hydroxyl group at the 2". Carbon: below are shown the sequences of two strands of dna from a protein-coding gene. The translation start site is included in the sequences shown. 5" a g g c g t a c c g c a g t g t c c t t a g c a t t a c g 3". 3" t c c g c a t g g c g t c a c a g g a a t c g t a a t g c 5". First reaction/ transesterification: one intron attaches itself to the adenosine branch point nucleotide.

Related Questions

1) Explain how the Ames test can be used to detect potential mutagens? What are the limitations of this test?

2) The base pair sequence of a region of a gene coding for a polypeptide is:

Strand A: 5â C A C C T A A C T T A C T C A G* C T T A C G A A G T C T A C C C A G C G 3â

Strand B: 3â G T G G A T T G A A T G A G T C* G A A T G C T T C A G A T G G G T C G C 5â

A.) If this gene codes for a polypeptide that contains three Tyrosines (Tyr), which strand is transcribed? (1 pt)

B). What is the nucleotide of the mRNA transcribed from this DNA? (Note: there is no âstartâ codon. Simply start from the âappropriateâ end). You MUST label the appropriate 5 â and 3â ends.

C.) What is the polypeptide sequence of the protein translated from this mRNA?

D.) Hydroxylamine (HA) affects the bold-faced (*) base pair and causes a mutation during DNA replication. What is the amino acid sequence of the polypeptide coded by the mutant gene?

1. The Pribnow Box is a

A. attenuator

B. enhancer

C. promoter

D. operator

2. Which of the following do not occur during post-transcriptional processing of RNA in eukaryotes?

A. the splicing of introns

B. addition of a poly-A at the 3' end

C. removal of introns

D. addition of a 7-methyl guanosine cap at the 5' end

3. In transcription, RNA polymerase binds at

A. a promoter

B. an operator

C. the oriC locus

D. an enhancer

4. The Central Dogma in genetics describes

A. the pattern of information flow in the cell

B. the pattern of chromosomal inheritance in populations

C. the role of mutations in disease

D. the role of promoters

5. Which of the following sequences of DNA could code for a stem-loop structure (only the template strand is shown)?

A. 3'--G-G-C-C-C-T-T-G-G-A-A-A-A-C-C-A-A-G-G-G-C-C--5'

B. 3'--G-G-C-C-C-T-T-G-G-A-A-A-A-G-G-T-T-C-C-C-G-G--5'

C. 3'--G-G-C-C-C-T-T-G-G-T-T-T-T-G-G-C-C-C-T-T-G-G--5'

D. none of the above

6. Removal of introns from RNA is by

A. primosomes

B. peptidases

C. galactosidases

D. spliceosomes

7. Which of the following represents the units for measuring the rate of sedimentation of a molecule/organelle in a sucrose gradient?

A. molecular weight

B. Svedberg units

C. map units

D. Daltons

8. During transcription, which strand is not read by the RNA polymerase?

A. coding strand

B. noncoding strand

C. template strand

D. terminator strand

9. The function of the rho protein is

A. to help terminate translation

B. to help RNA polymerase bind to the DNA

C. to help RNA polymerase find a promoter

D. to help terminate transcription

10. The unusual bases found in transfer RNAs

A. are added during transcription

B. are normal bases that have been modified after transcription

C. help the tRNAs to bind to DNA

D. ensure that the tRNA stays linear in solution

DNA Structure and Function LabReport

DNA Structure
Which two scientists are credited with discovering DNA?

Name the nitrogen bases that are purines.

Which nitrogen base pairs with thymine?

List the three components of a nucleotide.

DNA Replication
What is the purpose of DNA replication?

How many times does replication occur in the life of acell?

In the Lab, Exercise 2, the original strand on the left had thebases shown below. Input the new bases that correctly pair with theoriginal strand.

Original	New
C
A
G
T

RNA Structure

Describe the structure and function of RNA.

Refer to Exercise 3 and record the bases of the RNA strandproduced from the replicated DNA strand.

DNA	RNA
C
A
G
T

Record the differences between DNA and RNA in the tablebelow.

	DNA	RNA
Sugars
Bases
Strands

RNA Synthesis

The process of assembling RNA is called _________.

How is replication different from transcription?

Refer to Exercise 4. Write the letters for the base sequence ofmRNA in the spaces below DNA. Note that the order is reversed;start with the 3â end of the DNA strand and the 5â end of the mRNAstrand. Transcription is DNA to mRNA. Note RNA contains Uracilinstead of Thymine; There is no thymine in RNA.

DNA

3â

5â

mRNA

5â

3â

Protein Synthesis

What type of RNA provides amino acids to build polypeptidechains?

If a mRNA strand has the bases 5â CUC 3â, what amino acid willbe translated? Refer to the printable chart in Exercise 5.

Where in the cell does translation occur?

Genes

What could be the problem if there is a change in the basesequence of a gene as it is passed down to the offspring?

Give an example of a disorder that results from changes in theamino acid sequence.

What causes sickle cell anemia?

Gene Cloning

What is the function of a plasmid?

Print the document from Lab, Exercise 6. Complete the activityalong with the video demonstration. Sign, date, and take an imageof your final product and include with this lab report.

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BIO 2110 Lecture Notes - Lecture 1: Arginine, Tata Box, Helicase

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