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26 Dec 2018

1) Explain how the Ames test can be used to detect potential mutagens? What are the limitations of this test?

2) The base pair sequence of a region of a gene coding for a polypeptide is:

Strand A: 5’ C A C C T A A C T T A C T C A G* C T T A C G A A G T C T A C C C A G C G 3’

Strand B: 3’ G T G G A T T G A A T G A G T C* G A A T G C T T C A G A T G G G T C G C 5’

A.) If this gene codes for a polypeptide that contains three Tyrosines (Tyr), which strand is transcribed? (1 pt)

B). What is the nucleotide of the mRNA transcribed from this DNA? (Note: there is no “start” codon. Simply start from the “appropriate” end). You MUST label the appropriate 5 ‘ and 3’ ends.

C.) What is the polypeptide sequence of the protein translated from this mRNA?

D.) Hydroxylamine (HA) affects the bold-faced (*) base pair and causes a mutation during DNA replication. What is the amino acid sequence of the polypeptide coded by the mutant gene?

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Beverley Smith
Beverley SmithLv2
28 Dec 2018

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