1) Explain how the Ames test can be used to detect potential mutagens? What are the limitations of this test?
2) The base pair sequence of a region of a gene coding for a polypeptide is:
Strand A: 5â C A C C T A A C T T A C T C A G* C T T A C G A A G T C T A C C C A G C G 3â
Strand B: 3â G T G G A T T G A A T G A G T C* G A A T G C T T C A G A T G G G T C G C 5â
A.) If this gene codes for a polypeptide that contains three Tyrosines (Tyr), which strand is transcribed? (1 pt)
B). What is the nucleotide of the mRNA transcribed from this DNA? (Note: there is no âstartâ codon. Simply start from the âappropriateâ end). You MUST label the appropriate 5 â and 3â ends.
C.) What is the polypeptide sequence of the protein translated from this mRNA?
D.) Hydroxylamine (HA) affects the bold-faced (*) base pair and causes a mutation during DNA replication. What is the amino acid sequence of the polypeptide coded by the mutant gene?
1) Explain how the Ames test can be used to detect potential mutagens? What are the limitations of this test?
2) The base pair sequence of a region of a gene coding for a polypeptide is:
Strand A: 5â C A C C T A A C T T A C T C A G* C T T A C G A A G T C T A C C C A G C G 3â
Strand B: 3â G T G G A T T G A A T G A G T C* G A A T G C T T C A G A T G G G T C G C 5â
A.) If this gene codes for a polypeptide that contains three Tyrosines (Tyr), which strand is transcribed? (1 pt)
B). What is the nucleotide of the mRNA transcribed from this DNA? (Note: there is no âstartâ codon. Simply start from the âappropriateâ end). You MUST label the appropriate 5 â and 3â ends.
C.) What is the polypeptide sequence of the protein translated from this mRNA?
D.) Hydroxylamine (HA) affects the bold-faced (*) base pair and causes a mutation during DNA replication. What is the amino acid sequence of the polypeptide coded by the mutant gene?