Biochemistry 2280A Lecture Notes - Lecture 20: Pseudouridine, Antibiotics, Phenylalanine
17 Mar 2022
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2018-2019: all have similar structure, has to be recognized by ribosome, stem-loop structure due to internal base pairing, 3 loops, 4 stems, l shape in 3d, about 80 bases, non-conventional bases (ex. D, pseudouridine), added post-transcriptionally, play role in function. Heavily modified: bp still antiparallel, 2 single stranded regions, 3" acceptor site (cca), for amino acid, anticodon (base pairs with codon on mrna) Wobble: most organisms have less than 45 different trnas. how can all 61 codons be used: base pairing b/w anticodon and codon only requires matching at 2 positions, ex. Small subunit moves forward by one codon (towards 3" end) trna leaves e site, step 1 repeats. Correct charging of aa-trna synthetase and bp of anticodon and codon in a site of ribosome (a. a not directly recognized) Specificity from: synthetases, requirement for bp in a site of ribosome. Antibiotics and translation: many block bacterial translation, can be targeted at multiple places in process.
