BIO 203 Lecture Notes - Grow Light, Tuber, Xylem

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mag74

20 Nov 2022

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CSU

Department

Biology

Course

BIO 203

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Lab 6- vascular plant and functional anatomy: a. When looking at the results of the experiment, the bok choy leaf that was not cut had a faster rate of water movement in the time period of five minutes. This is shown by the sixteen two-centimeter sections we cut in the bok choy leaf after five minutes. For the uncut leaf, the dye showed in the stem all the way up into section twelve but did not show in sections fourteen and sixteen (lab results, 2022). The dye being present shows where the die had been. The first group had dye showing in the stem all the way through and into section twelve of the stem (lab results, 2022). Out of all the groups recorded, this group was the only one to show dye going into section sixteen of the stem for the cut leaf. The results for the uncut leaf were the same as the lab that my group did.

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Complete problem 25-1

Asses the following partial Preliminary Results section. Ensure that all the parts of a paragraph for the Preliminary Results section are provided and signaled (purpose or background of the experiment, experimental approach, results interpretation of the results, problem statement/unknown/logical next steps).

ELISA for secreted HBMS. Because HBMS is a secreted protein (46), we determined whether the HBMS protein produced in VSV - infected cells is secreted into the culture media. We detected HBMS in the media of VSV-HBMS infected cells but not in uninflected or recombinant WT VSV-infected cells by both western blot (data not shown) and qualitative ELISA. We found by quantitative ELISA that secreted HBMS levels reached 35 ng/ml in the media of VSV-HBMS infected BHK cells by 24 h post infection. Therefore, the HBMS produced in VSV- infected cells is correctly processed for secretion.

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This is a Peanut Allergy Study.

summarize the methods section and summarize the resultssection:

Methods:

Infants between the age of 4 and 11 months who had severeeczema, egg allergy, or both were selected. There were two studycohorts (those who showed no sensitivity to peanuts, while theother group showed a wheal measuring 1 to 4 mm in diameter). Anyinfant that had a wheal greater than 4 mm was not selected for thestudy based on the risk of having a serious reaction. Each of thetwo cohorts was randomly assigned to an experimental group. Thecontrol group was given a diet that avoided peanut foods and theexposure group was fed 6 grams of peanut-containing foods spacedout into at least 3 different exposures per week until they reachedthe age of 60 months. Compliance was measured through a foodfrequency questionnaire and through testing of bedding at the ageof 60 months for peanut particulate matter to verify theirexposure. The actual numbers of male versus female participants wasnever clearly stated. However, 64.8% of the avoidance group weremales compared to 55.2% of the consumption group

Results:

Infants who did not have any response to the initial skin testwere given 5 grams of peanut in a single dose. The immune IgEresponse was measured to determine if an allergy was present. Forparticipants who had shown a small reaction to peanut initiallywere given a double blind placebo controlled trial where a total of9.4 grams of peanut were administered in increments to determine ifa current allergy was present (this was done to keep theparticipants safe from a severe reaction).

Among the 542 infants in the group with a negative result on theinitial skin-prick test, 530 (97.8%) could be evaluated for theprimary. At 60 months of age, 13.7% of the avoidance group and 1.9%of the consumption group were allergic to peanuts; this absolutedifference in risk of 11.8 percentage points (95% confidenceinterval [CI], 3.4 to 20.3; P<0.001) represents an 86.1%relative reduction in the prevalence of peanut allergy.

All 98 children in the group with positive results on theinitial skin-prick test were evaluated. At 60 months of age, 35.3%of the avoidance group and 10.6% of the consumption group wereallergic to peanuts; the absolute difference in risk of 24.7percentage points (95% CI, 4.9 to 43.3; P=0.004) represents a 70.0%relative reduction in the prevalence of peanut allergy.

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ASEPTIC TECHNIQUE

Introduction Procedure < Include information that the readerwould need to repeat your experimental procedure. Observations andResults Gram stain result: (Experiment 1 and 2) Part I:Contamination Step 6: What color are the traffic lights after capsare removed? Step 7: What color are the traffic lights after capshave been left off and then replaced? Step 9: Appearance of tube:Step 12: Appearance of tube after overnight incubation: Part II:Aseptic Transfer Tube-to-Tube Test Was your aseptic transfersuccessful? (Contamination light and Inoculation light both green)Appearance of tube after overnight incubation: Discussion In theDiscussion section, answer the following questions: Whatprecautions are taken in a "wetlab" to prevent contamination?During the "Contamination" portion of the exercise, why was thecontamination not present when you first placed the phenol redglucose tube in the 37 degree incubator? After your sample had beenincubated for one day, why is the solution turbid? What did thecolor change of the medium indicate? What caused the contaminationof your sample? How successful were you with the aseptic technique?How many times did you need to repeat the experiment until you wereable to successfully inoculate your media without contamination?How important is sterile technique in a "wetlab?" Why? Conclusion< Within a few sentences, provide a concluding statement aboutthe results of your laboratory>

BACTERIAL ANATOMY Introduction Procedure < Includeinformation that the reader would need to repeat your experimentalprocedure. Do not include any observations or results in thissection> Observations and Results Gram stain result: (Experiment1 and 2) Discussion In the Discussion section, answer the followingquestions: Describe the morphology of each bacterium, e.g., cocci,rods, or spiral/curved. Describe the arrangement of the bacteria.Were the bacteria organized as singles, pairs, chains, clusters, oranother type of arrangement? Describe the Gram Stain procedure andhow to interpret the results. How does this apply to your Sample #1and Sample #2? Which stain is responsible for the results that yousee? Relate the results to cell structure. You can also useInternet resources. Make sure that you cite all external datasources, including your textbook. Indicate the terminology that isused for the cell anatomy and arrangement observed for the organismin Sample #1 and Sample #2.

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BIO 203 Lecture Notes - Grow Light, Tuber, Xylem

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