BIOL 2114L Lecture 12: Western Blotting Technique BIOL 2114 Lab - PhysioEx Exercise 12 Activity 4

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cpflora

24 Dec 2022

School

WGTC

Department

Biology

Course

BIOL 2114L

Professor

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Document Summary

You correctly answered: these are all correctly matched. Gel electrophoresis is used to separate proteins on the basis of. Western blotting can be used to test for. In this activity you will use western blotting to test for the presence of. You correctly answered: antibodies (like the indirect elisa). You correctly answered: hiv antigens separated by electrophoresis. You correctly answered: the constant region of the primary antibody. Patient b no no p55 no yes p31 no no p24 no yes. Negative control no yes yes no no yes yes no. You scored 100% by answering 4 out of 4 questions correctly. You correctly answered: come from the patient sample. A key di erence between the elisa and the western blot technique is that the. You correctly answered: western blot looks at separated antigens. The antibodies in a positive patient sample bind to the. You correctly answered: constant region of the enzyme-linked antibody and the antigen bound to the nitrocellulose strip.

Related Questions

Can someone answer the question and provide a short explanation? Thanks!

Which of the following statements is NOT correct regarding protein seperation techniques?

A) Size-exclusion chromatography works on the same principle as polyacrylamide gel electrophoresis

B) Specific antibodies are required for immunoblotting, antibody affinity chromatography as well as immunoprecipitation

C) Two-dimensional gel electrophoresis is conceptually the same as combining gel filtration chromatography and ion exchange chromatography

D) Western blotting is a useful technique to isolate proteins for use in downstream assays

amethystsquirrel762

Which of the following statements is NOT correct regarding protein separation techniques?

a) Size-exclusion chromatography works on the same principle as polyacrylamide gel electrophoresis.

b) Specific antibodies are required for immunoblotting, antibody affinity chromatography as well as immunoprecipitation.

c) Two-dimensional gel electrophoresis is conceptually the same as combining gel filtration chromatography and ion exchange chromatography.

d) Western blotting is a useful technique to isolate proteins for us in downstream assays.

apricotwallaby184

What are the differences and similarities of western blotting and northern blotting? (check all that apply) A) Both techniques require the separation of molecules to be detected by gel-electrophoresis first B) Western blotting is a technique that is commonly used to detect a protein of interest, while northern blotting is a technique that is commonly used to detect a mRNA of interest C) In western blotting, the proteins are separated horizontally, while in northern blotting, the proteins are separated vertically D) Western blotting allows the comparison of relative abundance of a protein of interest in different samples, while northern blotting allows detection of a protein of interest but not quantification E) Both techniques rely on a specific probe. In western blotting, specific antibody is used as a probe to detect a protein of interest, while in northern blotting, a specific oligo-nucleotides is used as a probe to detect a mRNA of interest

BIOL 2114L Lecture 12: Western Blotting Technique BIOL 2114 Lab - PhysioEx Exercise 12 Activity 4

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