Question

Can someone answer the question and provide a short explanation? Thanks!

Which of the following statements is NOT correct regarding protein seperation techniques?

A) Size-exclusion chromatography works on the same principle as polyacrylamide gel electrophoresis

B) Specific antibodies are required for immunoblotting, antibody affinity chromatography as well as immunoprecipitation

C) Two-dimensional gel electrophoresis is conceptually the same as combining gel filtration chromatography and ion exchange chromatography

D) Western blotting is a useful technique to isolate proteins for use in downstream assays