CHEM 130 Lecture Notes - Sunset Yellow Fcf, Spectrophotometry, Spectroscopy
Document Summary
This experiment was performed to determine the relationship between absorbance and molar concentration. To do this we had to use a spectrophotometer to measure the wavelengths that can pass through each dye sample. The sample we used is sunset yellow, which we diluted multiple times to get seven different molarities of the dye solution. After recording the data for each different sample, we determined the similar peak wavelengths they have, which was the lambda max of the sunset yellow dye. After analyzing the data, we were able to find the extinction coefficient using beer"s law. After some calculations, this coefficient allowed us to conclude a directly proportional relationship between absorbance and molar concentration. Objective: the purpose of this experiment is to calculate the extinction coefficient, which will allow us to see the relationship between absorbance and molar concentration of a solution. Claim: after analyzing the data, we found the extinction coefficient to be 22,700.