You ask your lab assistant to prepare 1L of solution Zeta at a concentration of 1 M. He comes up to you with a 0.5 M stock solution and asks for help in calculating how much to use. What do you tell him?
2. Illustrate, as on your first lab calculation handout, how you would make 3 serial 4-fold dilutions of an original stock solution. Indicate for each tube (you can set up a table beneath the diagram), including the original stock solution, the following information:
A. What is the n-fold dilution for each tube ?
B. What is the relative concentration for each tube ?
C. What is the actual concentration in each tube if the original concentration = 1 M ?
D. If the original tube has 400 cells per mL, how many cells per mL are in each dilution tube ?
E. If you pipetted 0.25 mL of the second dilution tube into a cuvette, how many cells would there be in the cuvette ?
You ask your lab assistant to prepare 1L of solution Zeta at a concentration of 1 M. He comes up to you with a 0.5 M stock solution and asks for help in calculating how much to use. What do you tell him?
2. Illustrate, as on your first lab calculation handout, how you would make 3 serial 4-fold dilutions of an original stock solution. Indicate for each tube (you can set up a table beneath the diagram), including the original stock solution, the following information:
A. What is the n-fold dilution for each tube ?
B. What is the relative concentration for each tube ?
C. What is the actual concentration in each tube if the original concentration = 1 M ?
D. If the original tube has 400 cells per mL, how many cells per mL are in each dilution tube ?
E. If you pipetted 0.25 mL of the second dilution tube into a cuvette, how many cells would there be in the cuvette ?
