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24 Jun 2018
PBSS medium contains 3% NaCl, 50 mM sucrose and 0.2 mM Ca(NO3)2 in phosphate buffered trace salts. Agar is added to 1.5% for solid media. You need to preapre 12 plates of PBSS agar. Each plate will be filled with 20 mL of warm agar. You have unsterile stock solutions of 100 mM Ca(NO3)2, and 10X phosphate buffered trace salts. Sucrose, NaCl and agar are crystalline or powdered solids. Sucrose has a molecular weight of 342 g/mol. Describe how you would prepare and sterilize the PBSS agar.
PBSS medium contains 3% NaCl, 50 mM sucrose and 0.2 mM Ca(NO3)2 in phosphate buffered trace salts. Agar is added to 1.5% for solid media. You need to preapre 12 plates of PBSS agar. Each plate will be filled with 20 mL of warm agar. You have unsterile stock solutions of 100 mM Ca(NO3)2, and 10X phosphate buffered trace salts. Sucrose, NaCl and agar are crystalline or powdered solids. Sucrose has a molecular weight of 342 g/mol. Describe how you would prepare and sterilize the PBSS agar.
Elin HesselLv2
25 Jun 2018
