Gene X has a point mutation resulting in a premature stop codon in the mRNA. Imagine one cell line with this mutant was wild type (for all non Gene X genes) while another had a mutation in the nonsense mediated decay (NMD) pathway in addition to the Gene X mutation. If proteins were extracted from each cell line and a western blot probing for Gene X was performed, what would we expect to see on the western blot?
a) The Gene X band from both cell lines will be the same size and intensity.
b) The Gene X band in the wild type cell will be darker but the same size as the NMD mutant cell line band.
c) The Gene X band in the wild type cell will be lighter but the same size as the NMD mutant cell line band.
d) The Gene X band in the wild type cell will be darker but a smaller size compared to the NMD mutant cell line band.
e) The Gene X band in the wild type cell will be lighter but a larger size compared to the NMD mutant cell line.
Choices e and d are incorrect so far
Gene X has a point mutation resulting in a premature stop codon in the mRNA. Imagine one cell line with this mutant was wild type (for all non Gene X genes) while another had a mutation in the nonsense mediated decay (NMD) pathway in addition to the Gene X mutation. If proteins were extracted from each cell line and a western blot probing for Gene X was performed, what would we expect to see on the western blot?
| a) | The Gene X band from both cell lines will be the same size and intensity. |
| b) | The Gene X band in the wild type cell will be darker but the same size as the NMD mutant cell line band. |
| c) | The Gene X band in the wild type cell will be lighter but the same size as the NMD mutant cell line band. |
| d) | The Gene X band in the wild type cell will be darker but a smaller size compared to the NMD mutant cell line band. |
| e) | The Gene X band in the wild type cell will be lighter but a larger size compared to the NMD mutant cell line. |
Choices e and d are incorrect so far
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Part B
Think about the DNA coding sequence of a gene. If an A were swapped for a T, what kind of mutation could it cause and why?
| a) It could cause a frameshift nonsense or frameshift missense mutation because it would change the reading frame of the codon triplet. | ||||||||||||||||||||||||||||
| b) It could cause a silent, missense, or nonsense mutation because those are the types that can be caused by a nucleotide-pair substitution like this one. | ||||||||||||||||||||||||||||
| c) It could cause a nonsense mutation because the sequence would no longer be the same, so the protein would be shorter and non-functional. | ||||||||||||||||||||||||||||
| d) It could cause a silent mutation because A and T are complementary to each other so it is not really a substitution mutation. Part C Why is a frameshift missense mutation more likely to have a severe effect on phenotype than a nucleotide-pair substitution missense mutation in the same protein?
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