Given the following DNA sequence and PCR primers, underline and label the annealing locations for the two primers and draw a box around the target sequence
Please underline and label the annealing location for the two primers and draw a box around the target sequence. Please explain how this is done
5â-AGTCGCTATTCGGATATCGCGCTAGTTCGATAGAGCACGAGCTCG-3â
3â-TCAGCGATAAGCCTATAGCGCGATCAAGCTATCTCGTGCTCGAGC-5â
Primer1: GTCGCTATTCG
Primer2: GAGCTCGTGCT
If you can answer these other questions, it will be appreciated but really need to understand the first question. Thank you
If you are using a DNA sequence from a related species to design primers for your species of interest, why is it better to design your primers to complement an exon?
Why is it better to use a 25-base primer than a 10-base primer? Why do you have to have a lower annealing temperature for a 10-base primer?
Given the following DNA sequence and PCR primers, underline and label the annealing locations for the two primers and draw a box around the target sequence
Please underline and label the annealing location for the two primers and draw a box around the target sequence. Please explain how this is done
5â-AGTCGCTATTCGGATATCGCGCTAGTTCGATAGAGCACGAGCTCG-3â
3â-TCAGCGATAAGCCTATAGCGCGATCAAGCTATCTCGTGCTCGAGC-5â
Primer1: GTCGCTATTCG
Primer2: GAGCTCGTGCT
If you can answer these other questions, it will be appreciated but really need to understand the first question. Thank you
If you are using a DNA sequence from a related species to design primers for your species of interest, why is it better to design your primers to complement an exon?
Why is it better to use a 25-base primer than a 10-base primer? Why do you have to have a lower annealing temperature for a 10-base primer?
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