Can someone please check and help me with these? Especially II and III?
I. Below is a DNA sequence that is a template strand and contains an Open reading frame for a gene:
3â TAA ATA CGT GAG GCA CTT CAA AGA CCG CAC ACT_ AAT TT 5â
5â AUU UAU GCA CUC CGU GAA GUU UCU GGC GUG UGA _ UUA AA 3â
a. Transcribe the DNA template into a strand of mRNA by typing the mRNA sequence below the template strand. Label the 5â and 3â end of the mRNA 5â AUU UAU GCA CUC CGU GAA GUU UCU GGC GUG UGA _ UUA AA 3â
b. Paste the mRNA below and do all 3 reading frames. Highlight all start codons green in all the reading frames. Highlight red the stop codons which are in frame with the start codons in all the 3 reading frames if they are present.
1) 5â AUU UAU GCA CUC CGU GAA GUU UCU GGC GUG UGA UUA AA 3â
2) 5â A UUU AUG CAC UCC GUG AAG UUU CUG GCG UGU GAU UAA A 3â
3) 5â AU UUA UGC ACU CCG UGA AGU UUC UGG CGU GUG AUU AAA 3â
c) In which reading frame is the ORF found? The second one. Since it has AUG.
d) Paste the mRNA showing the most likely ORF below and translate the codons. Label the 5â and 3â end of the mRNA and the N- and C-termini of the peptide.
5â A UUU AUG CAC UCC GUG AAG UUU CUG GCG UGU GAU UAA A 3â
MET HIS SER VAL LYS PHE LEU ARG CYS ASP STOP
N Terminus C Terminus
II. 3 pts. The base calling program made a mistake that you didnât catch and deleted the bolded G in the original template strand above. Paste the template DNA below and delete the G. Transcribe (show the codons) and translate the resulting sequence. Label the 5â and 3â ends of the DNA and mRNA and the N and C-termini of the peptide. Comment on the resulting peptide sequence.
5â AUUU AUG CAC UCC GUG AAG UUU CUG GCG UGU GAU UAA A â3
MET HIS SER VAL LYS PHE LEU ARG CYS ASP STOP
III. 3 pts. The base calling program made a mistake that you didnât catch and added an extra G after the bolded G in the original template strand above. Paste the template DNA below and insert the extra G. Transcribe (show the codons) and translate the resulting sequence. Label the 5â and 3â ends of the DNA and mRNA and the N and C-termini of the peptide. Comment on the resulting peptide sequence.
5â AUUU AUG CAC UCC GUG AAG UUU CUG GGG UGU GAU UAA A â3
MET HIS SER VAL LYS PHE LEU GLY CYS ASP STOP
Can someone please check and help me with these? Especially II and III?
I. Below is a DNA sequence that is a template strand and contains an Open reading frame for a gene:
3â TAA ATA CGT GAG GCA CTT CAA AGA CCG CAC ACT_ AAT TT 5â
5â AUU UAU GCA CUC CGU GAA GUU UCU GGC GUG UGA _ UUA AA 3â
a. Transcribe the DNA template into a strand of mRNA by typing the mRNA sequence below the template strand. Label the 5â and 3â end of the mRNA 5â AUU UAU GCA CUC CGU GAA GUU UCU GGC GUG UGA _ UUA AA 3â
b. Paste the mRNA below and do all 3 reading frames. Highlight all start codons green in all the reading frames. Highlight red the stop codons which are in frame with the start codons in all the 3 reading frames if they are present.
1) 5â AUU UAU GCA CUC CGU GAA GUU UCU GGC GUG UGA UUA AA 3â
2) 5â A UUU AUG CAC UCC GUG AAG UUU CUG GCG UGU GAU UAA A 3â
3) 5â AU UUA UGC ACU CCG UGA AGU UUC UGG CGU GUG AUU AAA 3â
c) In which reading frame is the ORF found? The second one. Since it has AUG.
d) Paste the mRNA showing the most likely ORF below and translate the codons. Label the 5â and 3â end of the mRNA and the N- and C-termini of the peptide.
5â A UUU AUG CAC UCC GUG AAG UUU CUG GCG UGU GAU UAA A 3â
MET HIS SER VAL LYS PHE LEU ARG CYS ASP STOP
N Terminus C Terminus
II. 3 pts. The base calling program made a mistake that you didnât catch and deleted the bolded G in the original template strand above. Paste the template DNA below and delete the G. Transcribe (show the codons) and translate the resulting sequence. Label the 5â and 3â ends of the DNA and mRNA and the N and C-termini of the peptide. Comment on the resulting peptide sequence.
5â AUUU AUG CAC UCC GUG AAG UUU CUG GCG UGU GAU UAA A â3
MET HIS SER VAL LYS PHE LEU ARG CYS ASP STOP
III. 3 pts. The base calling program made a mistake that you didnât catch and added an extra G after the bolded G in the original template strand above. Paste the template DNA below and insert the extra G. Transcribe (show the codons) and translate the resulting sequence. Label the 5â and 3â ends of the DNA and mRNA and the N and C-termini of the peptide. Comment on the resulting peptide sequence.
5â AUUU AUG CAC UCC GUG AAG UUU CUG GGG UGU GAU UAA A â3
MET HIS SER VAL LYS PHE LEU GLY CYS ASP STOP