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4 Mar 2018
1. Assume a mixture of GFP, cytochrome c and hemoglobin was separated using the same gel filtration column and conditions you used in lab. Based on the size of each protein, list them in order from smallest to largest expected partition coefficient value. 2. Justify your answer above. 3. Are any of the proteins larger than the exclusion limit? If so which ones? Concisely explain the expected value for partition coefficient for a protein larger than the exclusion limit.
1. Assume a mixture of GFP, cytochrome c and hemoglobin was separated using the same gel filtration column and conditions you used in lab. Based on the size of each protein, list them in order from smallest to largest expected partition coefficient value.
2. Justify your answer above.
3. Are any of the proteins larger than the exclusion limit? If so which ones? Concisely explain the expected value for partition coefficient for a protein larger than the exclusion limit.
Irving HeathcoteLv2
6 Mar 2018
