You receive data back from the Qubit showing that your total RNA concentration is 2.5 ug/mL. The sample run on the Qubit was diluted 100-fold, so you must correct for this to determine the actual concentration. For the same RNA sample, the Nanodrop gives you a value of 273 ng/uL.
1. How does the concentration from the Nanodrop compare to the concentration from the Qubit? SHOW your data and units conversions in your answer. Why do you think your concentrations from the Nanodrop and Qubit might be different?
2. If you need 1 ug of total RNA for the mRNA isolation step, what volume of your total RNA sample would you need (in uL) and which concentration should you use for this calculation and why?
You receive data back from the Qubit showing that your total RNA concentration is 2.5 ug/mL. The sample run on the Qubit was diluted 100-fold, so you must correct for this to determine the actual concentration. For the same RNA sample, the Nanodrop gives you a value of 273 ng/uL.
1. How does the concentration from the Nanodrop compare to the concentration from the Qubit? SHOW your data and units conversions in your answer. Why do you think your concentrations from the Nanodrop and Qubit might be different?
2. If you need 1 ug of total RNA for the mRNA isolation step, what volume of your total RNA sample would you need (in uL) and which concentration should you use for this calculation and why?
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