in a hypothetical experiment, we would like to insert gene Y fromhuman genomic DNA into a plasmid X using restriction enzymes I andII. where restriction enzyme I cuts the plasmid at only one site,and restriction enzyme II cuts the DNA at two sites.
which restriction enzyme,I or II, should we choose to cut plasmidX? explain why.
in a hypothetical experiment, we would like to insert gene Y fromhuman genomic DNA into a plasmid X using restriction enzymes I andII. where restriction enzyme I cuts the plasmid at only one site,and restriction enzyme II cuts the DNA at two sites.
which restriction enzyme,I or II, should we choose to cut plasmidX? explain why.
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View/perform/read ALL THREE of the following prior to answeringthe questions.
http://highered.mcgraw-hill.com/olcweb/cgi/pluginpop.cgi?it=swf::535::535::/sites/dl/free/0072437316/120078/micro10.swf::Stepsin Cloning a Gene (Links to an external site.)
http://www.discoverbiotech.com/wiki/-/wiki/Main/Applications ofCloning (Links to an external site.)
http://www.wiley.com/college/boyer/0470003790/animations/cloning/cloning.htm(Links to an external site.)
Skip to question text.
From the list below, which of the following is the most logicalsequence of steps for splicing foreign DNA into a plasmid andinserting the plasmid into a bacterium?
I. Transformbacteria with recombinant DNA molecule
II. Cutthe plasmid DNA using restriction enzymes
III. Extractplasmid DNA from bacterial cells
IV. Hydrogen-bondthe plasmid DNA to nonplasmid DNA fragments
V. Useligase to seal plasmid DNA to nonplasmid DNA
| IV, V, I, II, III |
| III, II, IV, V, I |
| III, IV, V, I, II |
| II, III, V, IV, I |
| I, II, IV, III, V |
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Plasmids (or vectors) are important in biotechnology becausethey are
| a vehicle for the insertion of recombinant DNA intobacteria. |
| surfaces for respiratory processes in bacteria. |
| recognition sites on recombinant DNA strands. |
| surfaces for protein synthesis in eukaryotic recombinants. |
| proviruses incorporated into the host DNA |
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Plasmids are put into bacterial cells by
| restriction enzymes |
| DNA ligase |
| binding of cohesive sticky ends |
| transformation |
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Restriction enzymes usually
| cut donor DNA evenly so smooth edges result |
| cut donor DNA but do not affect plasmids |
| make staggered cuts at specific sequences in DNA in both donorDNA and plasmid |
| are used in ligating plasmids into bacterial host cells |
| more than one of the above |
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After combining DNA fragments in a cloning experiment, ___ isused to covalently join the DNA segments.
| Restriction enzyme |
| DNA Ligase |
| Reverse transcriptase |
| DNA polymerase |
| Helicase |
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It is theoretically possible for a gene from any organism tofunction in any other organism. Why is this possible?
| All organisms have ribosomes. |
| All organisms have the same genetic code. |
| All organisms are made up of cells. |
| All organisms have similar nuclei. |
| All organisms have transfer RNA. |
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Skip to question text.
Assume that you are trying to insert a gene into a plasmid andsomeone gives you a DNA sample cut with restriction enzyme X. Thegene you wish to insert from the given sample has sites on bothends for cutting by restriction enzyme Y. You have a plasmid with asingle site for Y, but not for X. Your strategy should be to
| cut the plasmid with restriction enzyme X and insert thefragments cut with Y into the plasmid. |
| cut the plasmid with enzyme X and then insert the gene into theplasmid. |
| cut the DNA again with restriction enzyme Y and insert thesefragments into the plasmid cut with the same enzyme. |
| cut the plasmid twice with restriction enzyme Y and ligate thetwo fragments onto the ends of the human DNA fragments cut withrestriction enzyme X. |
| insert the fragments cut with X directly into the plasmidwithout cutting the plasmid. |
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Which of the following is/are false in regard to expressionplasmids (also called expression vectors)?
| They are used to make proteins using a cloned gene. |
| They contain a promotor. |
| They are the first plasmid type used to clone a gene. |
| They contain a terminator. |
| More than one of the above is false. |
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What is NOT a potential problem(s) associated with usingbacteria containing a cloned eukaryotic gene (e.g. a human gene) toproduce a functional protein?
| If the eukaryotic gene contains introns the bacteria will notremove them and the resulting amino acid sequence will be differentthat that made by a eukaryote. |
| The bacteria may not fold the protein correctly. |
| The bacteria may degrade the protein. |
| All of the above are potential problems. |
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Cloning allows for production of proteins in much larger amountsthan occurs in the cells from which the gene is isolated.
| True |
| False |
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Question 111 pts
Gene cloning is used to do all of the following except
| Make insulin |
| Making genetically identical animals (e.g. Dolly thesheep) |
| Make vaccines |
| Perform Gene Therapy |
| Making genetically engineered plants |
Flag this Question
In your
View/perform/read ALL THREE of the following prior to answeringthe questions.
http://highered.mcgraw-hill.com/olcweb/cgi/pluginpop.cgi?it=swf::535::535::/sites/dl/free/0072437316/120078/micro10.swf::Stepsin Cloning a Gene (Links to an external site.)
http://www.discoverbiotech.com/wiki/-/wiki/Main/Applications ofCloning (Links to an external site.)
http://www.wiley.com/college/boyer/0470003790/animations/cloning/cloning.htm(Links to an external site.)
Skip to question text.
From the list below, which of the following is the most logicalsequence of steps for splicing foreign DNA into a plasmid andinserting the plasmid into a bacterium?
I. Transformbacteria with recombinant DNA molecule
II. Cutthe plasmid DNA using restriction enzymes
III. Extractplasmid DNA from bacterial cells
IV. Hydrogen-bondthe plasmid DNA to nonplasmid DNA fragments
V. Useligase to seal plasmid DNA to nonplasmid DNA
| IV, V, I, II, III |
| III, II, IV, V, I |
| III, IV, V, I, II |
| II, III, V, IV, I |
| I, II, IV, III, V |
Flag this Question
Plasmids (or vectors) are important in biotechnology becausethey are
| a vehicle for the insertion ofrecombinant DNA into bacteria. |
| surfaces for respiratoryprocesses in bacteria. |
| recognition sites onrecombinant DNA strands. |
| surfaces for protein synthesisin eukaryotic recombinants. |
| proviruses incorporated intothe host DNA |
Flag this Question
Plasmids are put into bacterial cells by
| restriction enzymes |
| DNA ligase |
| binding of cohesive stickyends |
| transformation |
Flag this Question
Restriction enzymes usually
| cut donor DNA evenly so smoothedges result |
| cut donor DNA but do notaffect plasmids |
| make staggered cuts atspecific sequences in DNA in both donor DNA and plasmid |
| are used in ligating plasmidsinto bacterial host cells |
| more than one of theabove |
Flag this Question
After combining DNA fragments in a cloning experiment, ___ isused to covalently join the DNA segments.
| Restriction enzyme |
| DNA Ligase |
| Reverse transcriptase |
| DNA polymerase |
| Helicase |
Flag this Question
It is theoretically possible for a gene from any organism tofunction in any other organism. Why is this possible?
| All organisms haveribosomes. |
| All organisms have the samegenetic code. |
| All organisms are made up ofcells. |
| All organisms have similarnuclei. |
| All organisms have transferRNA. |
Flag this Question
Skip to question text.
Assume that you are trying to insert a gene into a plasmid andsomeone gives you a DNA sample cut with restriction enzyme X. Thegene you wish to insert from the given sample has sites on bothends for cutting by restriction enzyme Y. You have a plasmid with asingle site for Y, but not for X. Your strategy should be to
| cut the plasmid withrestriction enzyme X and insert the fragments cut with Y into theplasmid. |
| cut the plasmid with enzyme Xand then insert the gene into the plasmid. |
| cut the DNA again withrestriction enzyme Y and insert these fragments into the plasmidcut with the same enzyme. |
| cut the plasmid twice withrestriction enzyme Y and ligate the two fragments onto the ends ofthe human DNA fragments cut with restriction enzyme X. |
| insert the fragments cut withX directly into the plasmid without cutting the plasmid. |
Flag this Question
Which of the following is/are false in regard to expressionplasmids (also called expression vectors)?
| They are used to make proteinsusing a cloned gene. |
| They contain a promotor. |
| They are the first plasmidtype used to clone a gene. |
| They contain aterminator. |
| More than one of the above isfalse. |
Flag this Question
What is NOT a potential problem(s) associated with usingbacteria containing a cloned eukaryotic gene (e.g. a human gene) toproduce a functional protein?
| If the eukaryotic genecontains introns the bacteria will not remove them and theresulting amino acid sequence will be different that that made by aeukaryote. |
| The bacteria may not fold theprotein correctly. |
| The bacteria may degrade theprotein. |
| All of the above are potentialproblems. |
Flag this Question
Cloning allows for production of proteins in much larger amountsthan occurs in the cells from which the gene is isolated.
| True |
| False |
Flag this Question
Question 111 pts
Gene cloning is used to do all of the following except
| Make insulin |
| Making genetically identicalanimals (e.g. Dolly the sheep) |
| Make vaccines |
| Perform Gene Therapy |
| Making genetically engineeredplants |
Flag this Question
In your
