1.Which of the following is true of the blue/white screening technique?
Non-recombinant plasmids are white.
In recombinant plasmids, the LacZα is inactivated by the target gene.
The host strain must contain an intact lac operon.
All of the above
2. During the plasmid miniprep procedure, which of the following reagents was used to denature the nucleic acids into their single-stranded forms?
Sodium dodecyl sulfate
NaOH
Potassium Acetate
Isopropanol
3. Quantification of DNA using the Qubit system involved which of the following?
Measurement of visible light absorbance
Measurement of UV light absorbance
Measurement of fluorescence
None of the above
4. Which of the following occurs during the phenol/chloroform extraction technique?
Nucleic acids are denatured to their single-stranded versions.
Proteins are denatured.
The organic solvents each form a layer above and below the aqueous layer at the interface.
All of the above
5.What result would you expect if you omitted the ammonium acetate prior to ethanol precipitation of a DNA sample after a phenol chloroform extraction of a PCR reaction?
More Taq polymerase would be precipitated with the DNA.
Less of the DNA in the sample would be precipitated in the pellet.
More of the dNTPs would be precipitated in the pellet.
The pellet would be too difficult to resuspend in TE buffer.
1.Which of the following is true of the blue/white screening technique?
Non-recombinant plasmids are white.
In recombinant plasmids, the LacZα is inactivated by the target gene.
The host strain must contain an intact lac operon.
All of the above
2. During the plasmid miniprep procedure, which of the following reagents was used to denature the nucleic acids into their single-stranded forms?
Sodium dodecyl sulfate
NaOH
Potassium Acetate
Isopropanol
3. Quantification of DNA using the Qubit system involved which of the following?
Measurement of visible light absorbance
Measurement of UV light absorbance
Measurement of fluorescence
None of the above
4. Which of the following occurs during the phenol/chloroform extraction technique?
Nucleic acids are denatured to their single-stranded versions.
Proteins are denatured.
The organic solvents each form a layer above and below the aqueous layer at the interface.
All of the above
5.What result would you expect if you omitted the ammonium acetate prior to ethanol precipitation of a DNA sample after a phenol chloroform extraction of a PCR reaction?
More Taq polymerase would be precipitated with the DNA.
Less of the DNA in the sample would be precipitated in the pellet.
More of the dNTPs would be precipitated in the pellet.
The pellet would be too difficult to resuspend in TE buffer.
